Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.
نویسندگان
چکیده
The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 183 8 شماره
صفحات -
تاریخ انتشار 2001